Development of microsatellite markers and genetic diversity analysis for Pelodiscus sinensis

文献类型： 外文期刊

第一作者： Li, T.

作者： Li, T.;Zhao, J.;Li, W.;Shi, Y.;Hong, X. Y.;Zhu, X. P.;Li, T.;Hong, X. Y.;Zhu, X. P.

作者机构：

关键词： Pelodiscus sinensis;Chinese soft-shell turtle;Microsatellites;Genetic diversity

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 3 期

页码：

收录情况： SCI

摘要： Pelodiscus sinensis is a common freshwater soft-shell turtle found in China, and is an important aquaculture species. In this study, 20 polymorphic microsatellite primers were developed from the transcriptome. The genetic diversity of three populations of P. sinensis was evaluated, using 72 individuals. The number of alleles per locus ranged from 3 to 26. The observed and expected heterozygosities varied from 0.208 to 0.958, and from 0.302 to 0.963, respectively. The polymorphic information content varied from 0.283 to 0.953. No significant linkage disequilibrium was detected. These markers will be useful for future population genetic studies and molecular breeding of P. sinensis.

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