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Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense

文献类型：外文期刊

第一作者： Rahman, N. M. A.

作者： Rahman, N. M. A.;Fu, H.;Bai, H.;Jiang, F. W.;Liang, G.;Jiang, F. F.;Wu, Y.;Fu, H.;Qiao, H.;Jin, S.;Zhang, W.;Sun, S.;Gong, Y.;Xiong, Y.;Rahman, N. M. A.

作者机构：

关键词： Macrobrachium nipponense;Expression pattern;Feminization-1 homolog b (Fem1b);Crustacean;RT-qPCR;Post-larval

期刊名称：GENETICS AND MOLECULAR RESEARCH （影响因子：0.764；五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 2 期

页码：

收录情况： SCI

摘要： Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different developmental stages. Real-time quantitative reverse polymerase chain reaction (RT-qPCR) showed that the MnFem1b gene was expressed in all investigated tissues, with the highest expression level found in the testes. The results revealed that the MnFem1b gene might play roles in aspects of development of the male prawn phenotype. The RT-qPCR also revealed that MnFem1b mRNA expression was significantly increased at 10 days after metamorphosis. The expression levels in all investigated tissues showed a certain degree of sexually dimorphism, the expression levels in males were significantly higher than those in females (P < 0.05). Notably, the highest expression of MnFem1b was found in the testes. The expression of MnFem1b in different tissues indicates that it plays multiple biological functions in M. nipponense.

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