Efficacy of Trichoderma longibrachiatum SC5 Fermentation Filtrate in Inhibiting the Sclerotinia sclerotiorum Growth and Development in Sunflower

文献类型： 外文期刊

第一作者： Li, Enchen

作者： Li, Enchen;Zhu, Na;Zhang, Shuwu;Xu, Bingliang;Zhang, Shuwu;Liu, Lilong;Zhang, Aiqin;Liu, Lilong

作者机构：

关键词： Trichoderma; secondary metabolites; Sclerotinia sclerotiorum; growth and development inhibition; sclerotia production inhibition; species identification

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 1 期

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收录情况： SCI

摘要： Sclerotinia sclerotiorum is a destructive pathogen responsible for sunflower sclerotinia rot, resulting in substantial yield and economic losses worldwide. Trichoderma species have demonstrated the capacity to inhibit plant pathogen growth through the production of secondary metabolites. However, there are fewer recent studies focusing on the application of Trichoderma metabolites in inhibiting S. sclerotiorum growth and development and controlling sunflower sclerotinia rot disease. Our results showed that five Trichoderma strains (SC5, T6, TN, P6, and TS3) exhibited mycelial growth inhibition higher than 60% in dual culture assays out of the 11 tested strains. The Trichoderma SC5 fermentation filtrate exhibited superior efficacy compared to other strains, achieving a 94.65% inhibition rate of mycelial growth on S. sclerotiorum, 96% inhibition of myceliogenic germination of sclerotia, and 81.05% reduction in the oxalic acid content of S. sclerotiorum, while significantly increasing the cell membrane permeability. In addition, the Trichoderma SC5 fermentation filtrate significantly decreased the activities of polygalacturonase and pectin methyl-galacturonic enzymes and even caused S. sclerotiorum hyphae to swell, branch, twist, lyse, and inhibited the production and development of sclerotia. Moreover, the Trichoderma SC5 fermentation filtrate downregulated genes expression that associated with the growth and infection of S. sclerotiorum. The control efficacies of the protective and curative activities of the Trichoderma SC5 fermentation filtrate were 95.45% and 75.36%, respectively, on detached sunflower leaves at a concentration of 8 mg/mL. Finally, the Trichoderma SC5 was identified as Trichoderma longibrachiatum through morphological and phylogenetic analysis. Our research indicates that the T. longibrachiatum SC5 can be considered a promising biological control candidate against S. sclerotiorum and controlling the sunflower sclerotinia rot disease, both in vitro and in vivo.

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