Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration

文献类型： 外文期刊

第一作者： Wang Zhian

作者： Wang Zhian;Xiao Juanli;Zhang Anhong;Luo Xiaoli;Wang Peng;Wu Jiahe;Hu Guang;Wu Jiahe

作者机构：

关键词： Gossypium hirsutum; Somatic embryogenesis; OsLOL1; GhSOD1; GhAmy7

期刊名称：JOURNAL OF COTTON RESEARCH （ 影响因子：2.6； 五年影响因子：2.8 ）

ISSN： 2096-5044

年卷期： 2020 年 3 卷

页码：

收录情况： SCI

摘要： Background: Cotton somatic embryogenesis is difficult or rarely frequent to present, which has limited gene function identification and biotechnological utility. Here, we employed a rice key somatic embryogenesis-related gene, rice lesion simulating disease 1-like gene (OsLOL1), to develop transgenic cotton callus for evaluating its function in ectopic plants. Results: Overexpressing OsLOL1 can promote cotton callus to form embryogenic callus, not only shortening time but also increasing transition of somatic callus cells to embryogenic callus cells. And the regenerating plantlets per transgenic OsLOL1 embryogenic callus were significantly higher than those in the control transformed with empty vector. Analysis of physiological and biochemical showed that OsLOL1 can repress cotton superoxide dismutase 1 gene (GhSOD1) expression, possibly resulting in reactive oxidant species (ROS) accumulation in transgenic callus cells. And OsLOL1-overexpressed embryogenic callus exhibited higher alpha-amylase activity compared with the control, resulting from the promotion of OsLOL1 to cotton amylase 7 gene (GhAmy7) and GhAmy8 expression. Conclusion: The data showed that OsLOL1 could be used as a candidate gene to transform cotton to increase its somatic embryogenesis capacity, facilitating gene function analysis and molecular breeding in cotton.

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