Structurally manipulated antioxidant peptides derived from wheat bran: Preparation and identification

文献类型： 外文期刊

第一作者： Zhuang, Min

作者： Zhuang, Min;Wang, Anqi;Li, Gaoheng;Ke, Sheng;Wang, Xuanyu;Zhou, Zhongkai;Li, Jiaqing;Ning, Ming;Zhou, Zhongkai;Sheng, Zhanwu;Wang, Bing;Zhou, Zhongkai

作者机构：

关键词： Bioactive peptides; Antioxidant activity; Wheat Bran; Cellular model; Molecular docking; Keap1-Nrf2 interaction

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2024 年 442 卷

页码：

收录情况： SCI

摘要： Bioactive peptide's development is facing two challenges in terms of its lower yield and limited understanding of structurally orientated functionality. Therefore, peptides were prepared from wheat bran via a cocktail enzyme for achieving a higher level of hydrophobic amino acids than traditional method. The obtained peptides exhibited great antioxidant activities against H2O2-induced oxidative stress in HepG2 cells. Among them, 91 bioactive peptides were selected through the virtual screening, and their N -terminal and C -terminal contained many hydrophobic amino acids. Then the peptides with capacity to interact with Keap1 were identified by in silico simulation, because Keap1 acts as a sensor of redox insults. The results revealed that peptides DLDW and DLGL demonstrated the highest binding affinities, and a bridge was formed between Asp of DLGL and Arg415 of Klech domain, contributing to interfering Keap1-Nrf2 interaction. These findings implied a potential application of wheat bran peptides as nutraceuticals and health -promoting ingredients.

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