Genome-Wide Characterization and Sequence Polymorphism Analyses of Glycine max Fibrillin (FBN) Revealed Its Role in Response to Drought Condition

文献类型： 外文期刊

第一作者： Zafer, Muhammad Zeshan

作者： Zafer, Muhammad Zeshan;Khan, Zulqurnain;Ur Rehman, Shoaib;Zafer, Muhammad Zeshan;Tahir, Muhammad Hammad Nadeem;Khan, Zulqurnain;Bakhtavar, Muhammad Amir;Waheed, Ummara;Ur Rehman, Shoaib;Sajjad, Muhammad;Gao, Xiangkuo;Geng, Zhide;Siddique, Maria

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关键词： Glycine max; fibrillin (FBN); genome-wide identification; marker-assisted breeding; CAPS; drought

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

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年卷期： 2023 年 14 卷 6 期

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收录情况： SCI

摘要： The fibrillin (FBN) gene family is widely distributed in all photosynthetic organisms. Members of this gene family are involved in plant growth and development and their response to various biotic and abiotic stress factors. In this study, 16 members of FBN were identified in Glycine max and characterized by using different bioinformatics tools. Phylogenetic analysis classified FBN genes into seven groups. The presence of stress-related cis-elements in the upstream region of GmFBN highlighted their role in tolerance against abiotic stresses. To further decipher the function, physiochemical properties, conserved motifs, chromosomal localization, subcellular localization, and cis-acting regulatory elements were also analyzed. Gene expression analysis based on FPKM values revealed that GmFBNs greatly enhanced soybean drought tolerance and controlled the expression of several genes involved in drought response, except for GmFBN-4, GmFBN-5, GmFBN-6, GmFBN-7 and GmFBN-9. For high throughput genotyping, an SNP-based CAPS marker was also developed for the GmFBN-15 gene. The CAPS marker differentiated soybean genotypes based on the presence of either the GmFBN-15-G or GmFBN-15-A alleles in the CDS region. Association analysis showed that G. max accessions containing the GmFBN-15-A allele at the respective locus showed higher thousand seed weight compared to accessions containing the GmFBN-15-G allele. This research has provided the basic information to further decipher the function of FBN in soybean.

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