Biochemical characterization of tyrosine aminotransferase and enhancement of salidroside production by suppressing tyrosine aminotransferase in Rhodiola crenulata

文献类型： 外文期刊

第一作者： Liu, Xuechao

作者： Liu, Xuechao;Tang, Yueli;Zeng, Junlan;Liao, Zhihua;Lan, Xiaozhong;Qin, Jianbo;Lin, Min;Chen, Min;Lan, Xiaozhong

作者机构：

关键词： Salidroside; Tyrosine aminotransferase; 4-Hydroxyphenylpyruvate; Tyrosol; Metabolic engineering

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.645； 五年影响因子：5.749 ）

ISSN： 0926-6690

年卷期： 2021 年 173 卷

页码：

收录情况： SCI

摘要： Rhodiola crenulata is a traditional Tibetan medicinal plant mainly distributed in high altitude areas of Tibet. As the main extract of R. crenulata, salidroside has important medicinal activities in enhancing the body's immunity and resisting microwave radiation by influencing cell metabolism. Tyrosine is the starting amino acid precursor for salidroside biosynthesis. As an aromatic amino acid, tyrosine participates not only in salidroside biosynthesis, but also in other metabolite biosynthesis which might compete against salidroside biosynthetic pathway. In this study, we functionally identified a tyrosine aminotransferase in R. crenulata (RcTAT) that could convert tyrosine into 4-hydroxyphenylpyruvate (4-HPP). The Km and Vmax values of RcTAT for tyrosine were respectively 0.21 mM and 0.17 mu mol/min.mg under 9.0 pH and 39 degrees C. RNAi-mediated suppression of RcTAT expression markedly increased salidroside production in hairy root culture of R. crenulata. In summary, RcTAT is a key gene involved in the metabolic pathway that competes against salidroside biosynthesis, and suppressing its expression is a promising way to elevate salidroside production in planta.

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