Rapid detection of sweepoviruses through lateral flow dipstick-based recombinase polymerase amplification

文献类型： 外文期刊

第一作者： Wang, He

作者： Wang, He;Yang, Xiukun;Liu, Yonghua;Zhu, Guopeng;Wang, He;Yang, Xiukun;Liu, Yonghua;Zhu, Guopeng;Wang, He;Yang, Xiukun;Tuo, Decai;Zhou, Peng;Shen, Wentao;Wang, He;Yang, Xiukun;Tuo, Decai;Zhou, Peng;Shen, Wentao;Wang, He;Yang, Xiukun;Tuo, Decai;Zhou, Peng;Shen, Wentao;Wang, He;Yang, Xiukun;Tuo, Decai;Zhou, Peng;Shen, Wentao;Wang, He;Yang, Xiukun;Tuo, Decai;Zhou, Peng;Shen, Wentao

作者机构：

关键词： detection; sweepoviruses; recombinase polymerase amplification; lateral flow dipstick

期刊名称：ACTA VIROLOGICA （ 影响因子：1.827； 五年影响因子：1.632 ）

ISSN： 0001-723X

年卷期： 2022 年 66 卷 2 期

页码：

收录情况： SCI

摘要： Sweepoviruses represent a phylogenetic group of begomoviruses that cause significant sweet potato (Ipomoea batatas) production losses in various countries across the world. For rapid identification of sweepoviruses, we developed a technique based on isothermal recombinase polymerase amplification in conjunction with lateral flow dipsticks (RPA-LFD). The optimum reaction conditions for the RPA were 20 min incubation at 37 degrees C. The RPA-LFD specifically detected distinct sweepovirus species, with no other viruses infecting sweet potato causing a cross-reaction. The detection limit of the RPA-LFD was 1.0 x 10(4) copies of the target DNA molecule per reaction, and it exhibited a 10-fold greater sensitivity than the conventional PCR. Furthermore, when coupled with an alkaline polyethylene glycol-based crude genomic DNA extraction, the entire procedure was completed in 30 min without the use of any special instruments other than a water bath. Therefore, the RPA-LFD technique is a potential sweepovirus diagnostic tool that can be used in the field with fewer available resources.

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