文献类型： 外文期刊
第一作者： Liu, C.
作者： Liu, C.;Zhang, Z.;Liu, C.;Qin, X.;Liu, B.;Xu, X.;Zhang, Y.;Zhang, W.;Deng, A.
作者机构：
关键词： Aspergillus niger; juice processing; pectin lyase; Pichia pastoris; protein expression
期刊名称：LETTERS IN APPLIED MICROBIOLOGY （ 影响因子：2.813； 五年影响因子：3.086 ）
ISSN： 0266-8254
年卷期：
页码：
收录情况： SCI
摘要： A pectin lyase gene pnlzj5b from Aspergillus niger ZJ5 was identified and overexpressed successfully in Pichia pastoris. Recombinant PNLZJ5B exhibited high activity towards citrus pectin (150 U ml(-1)). Through further codon optimization, the expression efficiency of PNLZJ5B in P. pastoris increased to 3 center dot 5-fold (532/150 U ml(-1)). PNLZJ5B was purified by ultrafiltration, anion exchange and gel chromatography. It showed optimal activity and good stability at 58 degrees C and pH 4 center dot 5. PNLZJ5B activity improved with increasing degrees of methyl esterification of pectin. The K-m and V-max values were 0 center dot 81 mg ml(-1) and 372 center dot 8 mu mol min(-1) mg(-1), respectively. In addition, PNLZJ5B effectively decreased the viscosity of apple juice. Compared with commercial pectin lyase, PNLZJ5B obtained a higher juice volume. These favourable enzymatic properties of PNLZJ5B show potential utility in juice-processing applications and other food-related fields.
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