Development and characterization of wheat-Aegilops kotschyi 1U(k)(1A) substitution line with positive dough quality parameters

文献类型： 外文期刊

第一作者： JIANG, Yun

作者： JIANG, Yun;WANG, De-li;HAO, Ming;LIU, Deng-cai;JIANG, Yun;ZHANG, Jie;JIANG, Yun;WANG, De-li;HAO, Ming;LIU, Deng-cai

作者机构：

关键词： Aegilops kotschyi; common wheat; 1U(k)(1A) substitution line; HMW-GS; processing quality

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）

ISSN： 2095-3119

年卷期： 2023 年 22 卷 4 期

页码：

收录情况： SCI

摘要： Exploring novel high molecular weight glutenin subunits (HMW-GSs) from wild related species is a strategy to improve wheat processing quality. The objective of the present investigation was to identify the chromosomes of the wheat -alien introgression line N124, derived from the hybridization between Triticum aestivum with Aegilops kotschyi, and characterize the effects on quality-related traits. Fluorescence in situ hybridization karyotypes showed that N124 is a disomic 1U(k)(1A) substitution line. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed -phase high-performance liquid chromatography verified N124 expressed two HMW-GSs of the Ae. kotschyi parent. PacBio RNA sequencing and phylogenetic analysis confirmed that the two HMW-GSs were U(k)x and U(k)y. Compared to the wheat parent, the substitution line had no obvious agronomic defects except fewer grains per spike but improved several major quality parameters. It can be served as a donor or bridge material for wheat quality improvement.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序