High-level production of cordycepin by the xylose-utilising Cordyceps militaris strain 147 in an optimised medium

文献类型： 外文期刊

第一作者： Long, Liangkun

作者： Long, Liangkun;Liu, Zhen;Wang, Yizhou;Ding, Shaojun;Long, Liangkun;Ding, Shaojun;Lin, Qunying;Li, Chuanhua;Li, Chuanhua;Deng, Chunying;Lin, Qunying;Deng, Chunying

作者机构：

关键词： Cordyceps militaris; Cordycepin; Xylose; Medium optimisation; Transcriptome analysis

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2023 年 388 卷

页码：

收录情况： SCI

摘要： Cordycepin is an important active metabolite of Cordyceps militaris. Xylose, an attractive feedstock for producing chemicals through microbial fermentation, cannot be effectively utilised by many reported C. militaris strains. Herein, a xylose-utilising C. militaris strain 147 produced the highest level of cordycepin (3.03 g/L) in xylose culture. Xylose, alanine, and ammonium citrate were determined as the main affecting factors on the cordycepin production using a Plackett-Burman design. The combination of these factors was optimised using response surface methodology, and the maximal 6.54 g/L of cordycepin was produced by the fungus in the optimal medium. Transcriptome analysis revealed that xylose utilisation upregulated the transcriptional levels of genes participating in purine and energy metabolisms in the fungus, which may facilitate the formation of precursors for cordycepin biosynthesis. This investigation provides new insights into the efficient production of cordycepin and is conducive to the valorisation of biomass rich in xylose.

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