Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus

文献类型： 外文期刊

第一作者： Li Fenghui

作者： Li Fenghui;Gao Fei;Tan Jie;Fan Chaojing;Sun Huiling;Yan Jingping;Chen Siqing;Li Fenghui;Fan Chaojing;Wang Xiaojun

作者机构：

关键词： Apostichopus japonicus;gut;enzyme-producing microorganisms;16S rDNA-RFLP;aquaculture;probiotics

期刊名称：CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.068； 五年影响因子：0.983 ）

ISSN： 0254-4059

年卷期： 2016 年 34 卷 1 期

页码：

收录情况： SCI

摘要： Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopus japonicus gut. Thirty-nine strains that can produce at least one of the three digestive enzymes (protease, amylase, and cellulase) were qualitatively screened based on their extracellular enzyme-producing abilities. The enzyme-producing strains clustered into eight groups at the genetic similarity level of 100% by analyzing the restriction patterns of 16S rDNA amplified with Mbo I. Phylogenetic analysis revealed that 37 strains belonged to the genus Bacillus and two were members of the genus Virgibacillus. Enzyme-producing capability results indicate that the main enzyme-producing microflora in the A. japonicus gut was Bacillus, which can produce protease, amylase, and cellulase. Virgibacillus, however, can only produce protease. The high enzyme-producing capability of the isolates suggests that the gut microbiota play an important role in the sea cucumber digestive process.

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