Characterization and impact of olfactory cues from aphid honeydew microbes on Harmonia axyridis

文献类型： 外文期刊

第一作者： Liu, Jiahui

作者： Liu, Jiahui;Xu, Mengxian;Liu, Yong;Zhan, Yidi;Liu, Jiahui;Glacet, Lallie;Francis, Frederic;Liu, Yong;Zhan, Yidi;Liu, Jiahui;Xu, Pengjun;Ren, Guangwei

作者机构：

关键词： microbial volatile organic compounds; honeydew; ladybird; odorant-binding protein; biological control; behavioral manipulation

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：4.6； 五年影响因子：4.4 ）

ISSN： 0171-8177

年卷期： 2025 年 45 卷 2 期

页码：

收录情况： SCI

摘要： Microbial volatile organic compounds (mVOCs), produced by microorganisms associated with honeydew, play a crucial role in herbivore-natural enemy interactions. Nevertheless, the function of mVOCs on predatory ladybirds remains largely unknown. We hypothesized that the ladybird Harmonia axyridis (Coleoptera: Coccinellidae) could recognize and detect aphid honeydew-associated mVOCs for prey location. Our results showed that H. axyridis exhibited positive responses to the honeydew of Sitobion miscanthi (Hemiptera: Aphididae) and the mVOCs emitted by two honeydew-associated bacterial strains, Erwinia tasmaniensis MH4 and Klebsiella quasipneumoniae subsp. similipneumoniae MH6. A total of 16 mVOCs were detected by gas chromatography-mass spectrometry, with 3 mVOCs, namely 6-methylhept-5-en-2-one, 4-methylpentan-2-ol and 3-methylbutan-2-ol to be specific or significantly higher in attractive bacterial strains. Electroantennography and olfactory behavioral assays showed that H. axyridis prefers 6-methylhept-5-en-2-one and 4-methylpentan-2-ol. 3D modeling and molecular docking predicted that the H. axyridis olfactory binding proteins (HaxyOBP) HaxyOBP1 and HaxyOBP15 strongly interact with both compounds. Fluorescence competition assays further demonstrated that HaxyOBP15 exhibits a strong affinity for these attractive compounds. RNAi-mediated knockdown of HaxyOBP15 reduced H. axyridis' behavioral responses to 6-methylhept-5-en-2-one and 4-methylpentan-2-ol, confirming the critical role of HaxyOBP15 in detecting these mVOCs. We provide new insights on behavior of ladybirds in response to honeydew-associated mVOCs, contributing to research toward eco-friendly pest management methods targeting aphids.

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