Self-Responsive Fluorescence Aptasensor for Lactoferrin Determination in Dairy Products

文献类型： 外文期刊

第一作者： Liu, Hao

作者： Liu, Hao;Gao, Xibao;Qin, Hongwei;Yan, Mengmeng;Zhu, Chao;Qin, Hongwei;Yan, Mengmeng;Zhu, Chao;Li, Linsen;Qu, Feng

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关键词： aptamer; self-responsive; fluorescence aptasensor; lactoferrin detection

期刊名称：MOLECULES （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN：

年卷期： 2024 年 29 卷 13 期

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收录情况： SCI

摘要： In this study, a self-responsive fluorescence aptasensor was established for the determination of lactoferrin (Lf) in dairy products. Herein, the aptamer itself functions as both a recognition element that specifically binds to Lf and a fluorescent signal reporter in conjunction with fluorescent moiety. In the presence of Lf, the aptamer preferentially binds to Lf due to its specific and high-affinity recognition by folding into a self-assembled and three-dimensional spatial structure. Meanwhile, its reduced spatial distance in the aptamer-Lf complex induces a FRET phenomenon based on the quenching of 6-FAM by amino acids in the Lf protein, resulting in a turn-off of the fluorescence of the system. As a result, the Lf concentration can be determined straightforwardly corresponding to the change in the self-responsive fluorescence signal. Under the optimized conditions, good linearities (R-2 > 0.99) were achieved in an Lf concentration range of 2 similar to 10 mu g/mL for both standard solutions and the spiked matrix, as well as with the desirable detection limits of 0.68 mu g/mL and 0.46 mu g/mL, respectively. Moreover, the fluorescence aptasensor exhibited reliable recoveries (89.5-104.3%) in terms of detecting Lf in three commercial samples, which is comparable to the accuracy of the HPCE method. The fluorescence aptasensor offers a user-friendly, cost-efficient, and promising sensor platform for point-of-need detection.

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