Bacteriophage-mediated biosynthesis of MnO(2)NPs and MgONPs and their role in the protection of plants from bacterial pathogens

文献类型： 外文期刊

第一作者： Ogunyemi, Solabomi Olaitan

作者： Ogunyemi, Solabomi Olaitan;Abdallah, Yasmine;Ibrahim, Ezzeldin;Zhang, Yang;Ahmed, Temoor;Li, Bin;Abdallah, Yasmine;Bi, Ji'an A.;Wang, Fang;Yan, Chengqi Q.;Alkhalifah, Dalal Hussien M.;Hozzein, Wael N. N.;Xu, Lihui H.

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关键词： nanoparticles; bacteriophage; chlorophyll fluorescence; bacterial leaf blight; phytotoxicity; biosynthesis; Arabidopsis

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 14 卷

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收录情况： SCI

摘要： IntroductionXanthomonas oryzae pv. oryzae (Xoo) is the plant pathogen of Bacterial Leaf Blight (BLB), which causes yield loss in rice. MethodsIn this study, the lysate of Xoo bacteriophage X3 was used to mediate the bio-synthesis of MgO and MnO2. The physiochemical features of MgONPs and MnO(2)NPs were observed via Ultraviolet - Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Transmission/Scanning electron microscopy (TEM/SEM), Energy dispersive spectrum (EDS), and Fourier-transform infrared spectrum (FTIR). The impact of nanoparticles on plant growth and bacterial leaf blight disease were evaluated. Chlorophyll fluorescence was used to determine whether the nanoparticles application were toxic to the plants. ResultsAn absorption peak of 215 and 230 nm for MgO and MnO2, respectively, confirmed nanoparticle formation via UV-Vis. The crystalline nature of the nanoparticles was detected by the analysis of XRD. Bacteriological tests indicated that MgONPs and MnO(2)NPs sized 12.5 and 9.8 nm, respectively, had strong in vitro antibacterial effects on rice bacterial blight pathogen, Xoo. MnO(2)NPs were found to have the most significant antagonist effect on nutrient agar plates, while MgONPs had the most significant impact on bacterial growth in nutrient broth and on cellular efflux. Furthermore, no toxicity to plants was observed for MgONPs and MnO(2)NPs, indeed, MgONPs at 200 & mu;g/mL significantly increased the quantum efficiency of PSII photochemistry on the model plant, Arabidopsis, in light (& phi;PSII) compared to other interactions. Additionally, significant suppression of BLB was noted in rice seedlings amended with the synthesized MgONPs and MnO(2)NPs. MnO(2)NPs showed promotion of plant growth in the presence of Xoo compared to MgONPs. ConclusionAn effective alternative for the biological production of MgONPs and MnO(2)NPs was reported, which serves as an effective substitute to control plant bacterial disease with no phytotoxic effect.

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