An Efficient Approach for Large-Scale Production of Sialyglycopeptides from Egg Yolks

文献类型： 外文期刊

第一作者： Zou, Yang

作者： Zou, Yang;Wu, Zhigang;Liu, Xianwei;Gu, Guofeng;Xue, Mengyang;Wang, Peng George;Chen, Min;Zou, Yang;Wu, Zhigang;Liu, Xianwei;Gu, Guofeng;Xue, Mengyang;Wang, Peng George;Chen, Min;Chen, Leilei

作者机构：

关键词： Sialylglycopeptide; Egg yolk; Isolation; Graphitized carbon cartridge

期刊名称：JOURNAL OF CARBOHYDRATE CHEMISTRY （ 影响因子：1.667； 五年影响因子：1.397 ）

ISSN： 0732-8303

年卷期： 2012 年 31 卷 4-6 期

页码：

收录情况： SCI

摘要： Glycopeptides are very useful substrates for the synthesis of various complex glycoproteins and glycopeptides that are indispensable materials for the structural and functional investigation of glycoproteins. This report provides an efficient and time- and cost-saving strategy for the large-scale production of a sialylglycopeptide (SGP) that carries an intact N-linked disialyl-biantennary complex-type sialyloligosaccharide. Consequently, 680 mg of SGP was obtained from 100 egg yolks after phenol treatment, Sephadex G-50 gel filtration, and filtration through graphitized carbon cartridges. The chemical structure and the glycosylation site of the isolated SGP were identified by NMR and ESI-MS/MS, and the purity measured with HPLC was over 95%.

分类号： O6

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