Bacillus spore showcasing key antigens of African swine fever potentializes a promising oral vaccine candidate

文献类型： 外文期刊

第一作者： Wang, Xiaohua

作者： Wang, Xiaohua;Zhu, Tao;Shen, Hanwen;Li, Lian;Wang, Sihan;Yin, Xiulian;Tian, Baoxia;Zhang, Jinfeng;Li, Bin;Luo, Chuping;Fan, Baochao;Li, Bin;Luo, Chuping

作者机构：

关键词： African swine fever; Bacillus amyloliquefaciens; Spore surface display technology; Oral vaccine

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.3； 五年影响因子：5.1 ）

ISSN： 0175-7598

年卷期： 2025 年 109 卷 1 期

页码：

收录情况： SCI

摘要： African swine fever (ASF) remains a widespread and fatal infectious disease affecting both domestic pigs and wild boars around the world, and there is still no vaccine available for full protection. In recent years, Bacillus spores have been developed into effective vaccine carriers. Here, Bacillus amyloliquefaciens spores from strain CPLK1314 were used as a vaccine delivery system to display three highly antigenic proteins, p72, p30, and p54 of ASF, and their immune responses in BALB/c mice were evaluated. Our results demonstrated that the antibiotic-free Bacillus gene editing system constructed in this study can work effectively, and p72 and the fusion antigen p3054 were successfully expressed in the spore coat of CPLK1314 by fusing with CotB and CotG, respectively. Further animal experiments showed that the recombinant spores Delta Ba72 and Delta Ba3054 exhibited good immunogenicity, which induced high levels of antigen-specific serum IgG, proliferation of splenic lymphocytes, secretion of immune-related factors, as well as a significant increase in the proportion of T lymphocytes CD3+ CD4+ and CD3+ CD8+, and B lymphocytes B220+ CD19+. Interestingly, when providing the same number of spores, a 1:1 mixture of the two, Delta Ba72 + 3054, could simultaneously trigger similar levels of both p72 and p30 antibodies without cross-affecting production levels. Therefore, based on the CRISPR-Cas9 continuous gene editing system constructed in this study, the simultaneous co-expression of one antigen species or synergistic expression of multiple antigens at different spatial positions of B. amyloliquefaciens spores might make it a promising candidate for an ASF oral vaccine.Key points center dot Bacillus amyloliquefaciens spore is an ideal and effective vaccine delivery system center dot Bacillus spore showcasing key antigens of ASF is a promising oral vaccine candidate center dot The Bacillus vaccine can trigger effective protective responses in animalsKey points center dot Bacillus amyloliquefaciens spore is an ideal and effective vaccine delivery system center dot Bacillus spore showcasing key antigens of ASF is a promising oral vaccine candidate center dot The Bacillus vaccine can trigger effective protective responses in animalsKey points center dot Bacillus amyloliquefaciens spore is an ideal and effective vaccine delivery system center dot Bacillus spore showcasing key antigens of ASF is a promising oral vaccine candidate center dot The Bacillus vaccine can trigger effective protective responses in animals

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