Enhancing the Production of Xenocoumacin 1 in Xenorhabdus nematophila CB6 by a Combinatorial Engineering Strategy

文献类型： 外文期刊

第一作者： Qin, Youcai

作者： Qin, Youcai;Jia, Fenglian;Zheng, Xiaobing;Li, Xiaohui;Duan, Jiaqi;Li, Beibei;Shen, Hongfei;Yang, Xiufen;Ren, Jie;Li, Guangyue;Qin, Youcai;Shen, Hongfei

作者机构：

关键词： Xenorhabdus nematophila; xenocoumacin1; constitutive promoters; metabolic engineering

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 23 期

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收录情况： SCI

摘要： Xenocoumacin 1 (Xcn1) is an excellent antimicrobial naturalproductagainst Phytophthora capsici. However,the commercial development of Xcn1 is hindered by the low yield, whichresults in high application costs. In this study, multiple metabolicstrategies, including blocking the degradation pathway, promoter engineering,and deletion of competing biosynthetic gene clusters, were employedto improve the production of Xcn1, which was increased from 0.07 to0.91 g/L. The formation of Xcn1 reached 1.94 g/L in the TB mediumwith the final strain T3 in a shake flask and further reached 3.52g/L in a 5 L bioreactor, which is the highest yield ever reported.The engineered strain provides a valuable platform for productionof Xcn1, and the possible commercial development of the biofungicide.We anticipate that the metabolic engineering strategies utilized inthis study and the constructed constitutive promoter library can bewidely applied to other bacteria of the genera Xenorhabdus and Photorhabdus.

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