Metabolic engineering of Paenibacillus polymyxa for effective production of 2,3-butanediol from poplar hydrolysate

文献类型： 外文期刊

第一作者： Zhang, Jikun

作者： Zhang, Jikun;Liu, Haiyang;Li, Min;Wang, Zhongyue;Lin, Rongshan;Liu, Kai;Wang, Chengqiang;Zhao, Jianzhi;Zhao, Jianzhi;Fu, Quanbin;Dai, Li;Zhu, Xueming;Zhang, Jikun;Gu, Wei;Wang, Chengqiang

作者机构：

关键词： Renewable fuel; Omics analysis; Metabolic pathway; Raw poplar wood hydrolysates

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2024 年 392 卷

页码：

收录情况： SCI

摘要： 2,3-Butanediol is an essential renewable fuel. The synthesis of 2,3-butanediol using Paenibacillus polymyxa has attracted increasing attention. In this study, the glucose-derived 2,3-butanediol pathway and its related genes were identified in P. polymyxa using combined transcriptome and metabolome analyses. The functions of two distinct genes ldh1 and ldh3 encoding lactate dehydrogenase, the gene bdh encoding butanediol dehydrogenase, and the spore-forming genes spo0A and spoIIE were studied and directly knocked out or overexpressed in the genome sequence to improve the production of 2,3-butanediol. A raw hydrolysate of poplar wood containing 27 g/L glucose and 15 g/L xylose was used to produce 2,3-butanediol with a maximum yield of 0.465 g/g and 93 % of the maximum theoretical value, and the total production of 2,3-butanediol and ethanol reached 21.7 g/L. This study provides a new scheme for engineered P. polymyxa to produce renewable fuels using raw poplar wood hydrolysates.

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