Shinella oryzae sp. nov., a novel zearalenone-resistant bacterium isolated from rice paddy soil

文献类型： 外文期刊

第一作者： Gao, Ruyu

作者： Gao, Ruyu;Zheng, Juan;Lu, Wei;Ke, Xiubin;Chen, Ming;Lin, Min;Zhang, Wei;Zhou, Zhengfu

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关键词： Shinella oryzae sp; Rhizosphere soil; Zearalenone resistance

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.158； 五年影响因子：2.745 ）

ISSN： 0003-6072

年卷期： 2022 年 115 卷 5 期

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收录情况： SCI

摘要： A novel bacterium, designated Z-25( T), was isolated from a rice paddy rhizosphere soil sample from Wuchang County, China. The Z-25( T) strain is gram-negative, rod-shaped, non-spore-forming, aerobic, motile by unipolar flagella and straw white in color. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain Z-25 belongs to the genus Shinella, and the closest members are Shinella zoogloeoides ATCC 19623( T) with 98.58% similarity, S. kummerowiae CCBAU 25,048 T (98.03%) and S. granuli Ch06 (T) (97.37%). The average nucleotide identity and in silico DNA-DNA hybridization values between strain Z-25( T) and the closest members were less than 85.29% and 28.70%, respectively. The predominant fatty acids were the sums of features comprising C-18:1 omega 7c and/or C-18:1 omega 6c (34.62%), C-18:1 omega 7c -11-methyl (20.48%), and C-19:0 cyclo omega 8c (18.19%). The only respiratory quinone was ubiquinone-10, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Additionally, a genome analysis showed that Z-25( T) presented potential functional genes related to the degradation of zearalenone (ZEN). An HPLC analysis indicated that Z-25( T) could remove 74.13% of 10 mg/L ZEN after 144 h at 30 degrees C. Therefore, based on phenotypic, chemotaxonomic, phylogenetic and genotypic analyses, strain Z-25( T) represents a novel species in the genus Shinella, for which the name Shinella oryzae sp. nov. is proposed. The type strain is Z-25( T) (= GDMCC 1.2424( T) = KCTC 82660( T)).

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