Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis

文献类型： 外文期刊

第一作者： Luo, Zhengzhong

作者： Luo, Zhengzhong;Du, Zhenlong;Huang, Yixin;Zhou, Tao;Ma, Li;Yao, Xueping;Shen, Liuhong;Yu, Shumin;Cao, Suizhong;Yong, Kang;Du, Zhenlong;Yan, Zuoting

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关键词： ketosis; dairy cows; inflammatory biomarkers; tryptophan metabolism

期刊名称：METABOLITES （ 影响因子：4.1； 五年影响因子：4.5 ）

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年卷期： 2023 年 13 卷 3 期

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收录情况： SCI

摘要： Dairy cows with ketosis have high circulating beta-hydroxybutyric acid (BHBA) concentrations alongside which inflammation is concomitantly developed. Tryptophan (Trp) is an essential amino acid that participates in the regulation of the inflammatory response. However, the association between Trp metabolism and inflammation in dairy cows with ketosis remains unclear. Therefore, blood samples from healthy (n = 10) and ketotic (n = 10) primiparous dairy cows were collected at the calving date and the day of ketosis diagnosis (7 days in milk (7 DIM)). Serum levels of non-esterified fatty acids (NEFA), BHBA, haptoglobin (HP), serum amyloid A (SAA), lipopolysaccharide, and cortisol were analyzed. Tryptophan and its metabolites were quantified using liquid chromatography-tandem mass spectrometry. At 7 DIM, the concentrations of NEFA, BHBA, HP, and SAA were higher and the levels of Trp, kynurenine (KYN), indoleacetic acid, indole-3-lactic acid, and 3-indoxyl sulfate were lower in the dairy cows with ketosis compared with those in the healthy cows. However, the KYN/Trp and melatonin/Trp ratios increased in the cows with ketosis. At the calving date, the serum lipopolysaccharide levels did not differ between the healthy and ketotic cows, whereas the levels of NEFA, HP, and cortisol increased in the ketotic cows. Correlation analysis showed that Trp deficiency and elevated Trp metabolism in the dairy cows occurred during ketosis. Overall, our results suggest that abnormal Trp metabolism may contribute to the pathogenesis of ketosis.

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