Structural analysis of receptor-like kinase SOBIR1 reveals mechanisms that regulate its phosphorylation-dependent activation

文献类型： 外文期刊

第一作者： Wei, Xue

作者： Wei, Xue;Wang, Yulu;Gu, Tianyi;Yu, Haiyan;Wang, Fengzhong;Xin, Fengjiao;Zhang, Su;Steinmetz, Gabryel;Gu, Yangnan;Steinmetz, Gabryel;Gu, Yangnan;Guo, Guoguang;Liu, Xin;Fan, Shilong

作者机构：

关键词： LRR-RLK; SOBIR1; crystal structure; unusual architecture; autophosphorylation; stepwise activation

期刊名称：PLANT COMMUNICATIONS （ 影响因子：8.625； 五年影响因子：8.625 ）

ISSN： 2590-3462

年卷期： 2022 年 3 卷 2 期

页码：

收录情况： SCI

摘要： Plant leucine-rich repeat (LRR) receptor-like kinases (RLKs) and LRR receptor-like proteins (RLPs) comprise a large family of cell surface receptors that play critical roles in signal perception and transduction. Both LRR-RLKs and LRR-RLPs rely on regulatory LRR-RLKs to initiate downstream signaling pathways. NASE 3 (BAK1/SERK3) and SUPPRESSOR OF BIR1-1 (SOBIR1) are important and extensively studied regulatory LRR-RLKs with distinct functions. Although the regulatory mechanism of BAK1 activation has been studied in detail, the activation mechanism of SOBIR1 remains poorly understood. Here, the crystal structures of the catalytically inactive kinase domain of SOBIR1 (SOBIR1-KD) from Arabidopsis thaliana were determined in complexes with AMP-PNP and Mg2+. The results show that SOBIR1-KD contains a uniquely long 03-ocC loop and adopts an Src-like inactive conformation with an unusual architecture at the activation segment, which comprises three helices. Biochemical studies revealed that SOBIR1 is transphosphorylated by BAK1 following its autophosphorylation via an intermolecular mechanism, and the phosphorylation of Thr529 in the activation segment and the 03-ocC loop are critical for SOBIR1 phosphorylation. Further functional analysis confirmed the importance of Thr529 and the 03-ocC loop for the SOBIR1-induced cell death response in Nicotiana benthamiana. Taken together, these findings provide a structural basis for the regulatory mechanism of SOBIR1 and reveal the important elements and phosphorylation events in the special stepwise activation of SOBIR1-KD, the first such processes found in regulatory LRR-RLKs.

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