Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus

文献类型： 外文期刊

第一作者： Shi, Haoran

作者： Shi, Haoran;Wang, Jiabin;Liu, Chongxi;Li, Lei;Dong, Zhuoxu;Ye, Lan;Wang, Xiangjing;Xiang, Wensheng;Shi, Haoran;Wang, Jiabin;Li, Shanshan;Li, Lei;Dong, Zhuoxu;Ye, Lan;Zhang, Yanyan;Xiang, Wensheng

作者机构：

关键词： Guvermectin; Biopesticide; Regulatory mechanisms; Functional interaction; High-yield strategy

期刊名称：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY （ 影响因子：4.4； 五年影响因子：4.5 ）

ISSN： 2405-805X

年卷期： 2025 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Guvermectin, a purine nucleoside natural product produced by the genus Streptomyces, has recently been registered as a new biopesticide to boost rice yield. Despite its economic and agricultural significance, the regulatory mechanisms of guvermectin biosynthesis remain essentially unknown, hindering industrial production and widespread agricultural application. Here, we examined the roles of two LacI family regulators, gvmR and gvmR2, located within and adjacent to the guvermectin biosynthesis cluster, respectively, in guvermectin production in Streptomyces caniferus NEAU6. GvmR activated the expression of the guvermectin cluster by binding to the promoters of gvmR, gvmA, and O1, while GvmR2 repressed the guvermectin cluster via competitive binding to promoters containing GvmR-binding sites, specifically, a 14-bp palindromic sequences: 5 '-RTCATWCGYATGAY-3 ' (R = G/A, W = A/T, Y = T/C). Moreover, GvmR indirectly activates the expression of gvmR2 while GvmR2 feedback inhibits gvmR transcription, suggesting a functional interaction between the two regulators for coordinating guvermectin production. Overexpression of gvmR via the T7 RNA polymerase-T7 promoter system in the gvmR2 mutant significantly elevated guvermectin production by 125 % (from 631 mg L-1 to 1422 mg L-1), compared to the parental strain NEAU6. This suggested that combinatorial manipulation of gvmR and gvmR2 is useful for improving guvermectin production. These findings enrich our knowledge of the regulatory network for guvermectin biosynthesis, and offer key targets and effective strategies for high-titer guvermectin production.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序