The KL system in wheat permits homoeologous crossing over between closely related chromosomes

文献类型： 外文期刊

第一作者： Fan, Chaolan

作者： Fan, Chaolan;Li, Yazhou;Liu, Dengcai;Hao, Ming;Fan, Chaolan;Lukaszewski, Adam J.;Fan, Chaolan;Sun, Jiaojiao;Chen, Hong;Li, Liqiong;Zhang, Lanyue;Chen, Xue;Ning, Shunzong;Yuan, Zhongwei;Jiang, Bo;Chen, Xuejiao;Liu, Dengcai;Luo, Jiangtao

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关键词： ph-like gene; phKL; Homoeologous recombination; Homoeology

期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）

ISSN： 2095-5421

年卷期： 2023 年 11 卷 3 期

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收录情况： SCI

摘要： The Chinese wheat landrace Kaixianluohanmai (KL) expresses the ph-like phenotype. A major QTL, QPh. sicau-3A (syn. phKL), responsible for this effect has been mapped to chromosome arm 3AL. This study pre-sents some characteristics of homoeologous pairing and recombination induced by phKL. In KL haploids, the level of homoeologous pairing was elevated relative to Ph1 Chinese Spring (CS) haploids. There was a clear preference for A-D pairing and less frequent for A-B and B-D, reflecting the higher levels of affinity between genomes A and D in wheat. The characteristics of pairing were affected by temperature and magnesium ion supplementation. The suitability of phKL for chromosome engineering was tested on three pairs of homoeologues: 2Sv-2B, 2Sv-2D, and 2RL-2BL. The recombination rates were 1.68%, 0.17%, and 0%, respectively. The phKL locus in KL induced a moderate level of homoeologous chromosome pair -ing and recombination when the Ph1 locus of wheat was present, both in wheat haploids and hexaploids. The Ph1-imposed criteria for chromosome pairing and crossing over were relaxed to some degree, per -mitting homoeologous crossing over but only between closely related chromosomes; there was no cross -ing over between more differentiated chromosomes. Therefore, the phKL system (QPh.sicau-3A) can be a useful tool in chromosome engineering of wheat to transfer genes from closely related species with the benefit of reduced genomic chaos generated by the ph1b mutation. & COPY; 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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