The METHYLTRANSFERASE B-SERRATE interaction mediates the reciprocal regulation of microRNA biogenesis and RNA m6A modification

文献类型： 外文期刊

第一作者： Bai, Haiyan

作者： Bai, Haiyan;Dai, Yanghuan;Zhou, Yiming;Wang, Xiangying;Chen, Jingjing;Jiao, Yuzhe;Du, Chang;Huang, Zhuoxi;Xie, Yuting;Guo, Xiaoyu;Ling, Yongqing;Zhang, Zhonghui;Fan, Panting;He, Shengbo;Lang, Xiaoqiang;Ling, Yongqing;Liu, Qi;Lang, Xiaoqiang;Ling, Yongqing;Liu, Qi;Deng, Yizhen

作者机构：

关键词： liquid-liquid phase separation; microRNA biogenesis; MTB; RNA m(6)A modification; SE

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.3； 五年影响因子：10.8 ）

ISSN： 1672-9072

年卷期： 2024 年 66 卷 12 期

页码：

收录情况： SCI

摘要： In eukaryotes, RNA N-6-methyladenosine (m(6)A) modification and microRNA (miRNA)-mediated RNA silencing represent two critical epigenetic regulatory mechanisms. The m(6)A methyltransferase complex (MTC) and the microprocessor complex both undergo liquid-liquid phase separation to form nuclear membraneless organelles. Although m(6)A methyltransferase has been shown to positively regulate miRNA biogenesis, a mechanism of reciprocal regulation between the MTC and the microprocessor complex has remained elusive. Here, we demonstrate that the MTC and the microprocessor complex associate with each other through the METHYLTRANSFERASE B (MTB)-SERRATE (SE) interacting module. Knockdown of MTB impaired miRNA biogenesis by diminishing microprocessor complex binding to primary miRNAs (pri-miRNAs) and their respective MIRNA loci. Additionally, loss of SE function led to disruptions in transcriptome-wide m(6)A modification. Further biochemical assays and fluorescence recovery after photobleaching (FRAP) assay indicated that SE enhances the liquid-liquid phase separation and solubility of the MTC. Moreover, the MTC exhibited enhanced retention on chromatin and diminished binding to its RNA substrates in the se mutant background. Collectively, our results reveal the substantial regulatory interplay between RNA m(6)A modification and miRNA biogenesis.

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