Molecular characterization, expression following cold stress, and functional characterization of YB-1 gene in the spotted sea bass (Lateolabrax maculatus)

文献类型： 外文期刊

第一作者： Wang, Zhen

作者： Wang, Zhen;Wang, Jing;Mu, Weijie;Liang, Liqun

作者机构：

关键词： YB-1; Lateolabrax maculatus; Cloning; Expression; Overexpression

期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.953； 五年影响因子：2.94 ）

ISSN： 0967-6120

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收录情况： SCI

摘要： Existing data indicate that YB-1, a gene member of the cold chock domain family, is regulated by cold stress in many species. However, information regarding YB-1 in fish is limited. To gain more insight into the potential biological function of YB-1 after cold stress, we have cloned YB-1 of the spotted sea bass (Lateolabrax maculatus) and studied the expression at transcriptional level of the LmYB-1 gene under low temperature. The cloned LmYB-1 cDNA (1454 bp) includes a 900-bp open-reading frame that encodes a 300-residue protein and contains C-terminal, CSD, and N-terminal domains. The results of real-time quantitative PCR showed that LmYB-1 was expressed in all tested tissues, with the highest expression in liver. Within liver, brain, and heart, LmYB-1 mRNA expression increased during cold stressed fish, compared to control fish. No significant induces in the expression of LmYB-1 were recorded in muscle, except at 10 degrees C during 24 h cold stress. Overexpression of LmYB-1 in CIK cells caused the upregulation of the expression of Hsc70, SOD1, and SOD2. Our results provided new insights into the LmYB-1 expression during cold stress and warranted further investigation into this potentially important protein in improving fish farming at low temperature.

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