The glutathione S-transferase (PxGST2L) may contribute to the detoxification metabolism of chlorantraniliprole in Plutella xylostella(L.)

文献类型： 外文期刊

第一作者： Yin, Fei

作者： Yin, Fei;Lin, Qingsheng;Wang, Xiaoxiang;Li, Zhenyu;Feng, Xia;Shabbir, Muhammad Zeeshan;Yin, Fei;Lin, Qingsheng;Wang, Xiaoxiang;Li, Zhenyu;Feng, Xia

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关键词： Plutella xylostella (L; ); Chlorantraniliprole; Insecticide resistance; 2-DE; RNAi

期刊名称：ECOTOXICOLOGY （ 影响因子：2.823； 五年影响因子：2.958 ）

ISSN： 0963-9292

年卷期： 2021 年 30 卷 6 期

页码：

收录情况： SCI

摘要： The diamondback moth (Plutella xylostella L.), is an economic pest of cruciferous plants worldwide, which causes great economic loss to cruciferous plants production. However, the pest has developed resistance to insecticides. One of such insecticides is chlorantraniliprole. The study of the mechanisms underlying resistance is key for the effective management of resistance. In this study, a comparative proteomics approach was used to isolate and identify various proteins that differed between chlorantraniliprole-susceptible and -resistant strains of P. xylostella. Eleven proteins were significantly different and were successfully identified by MALDI-TOF-MS. Metabolism-related proteins accounted for the highest proportion among the eleven different proteins. The function of the PxGST2L protein was validated by RNAi. Knockdown of PxGST2L reduced the GST activity and increased the toxicity of chlorantraniliprole to the diamondback moth. The resistance ratio of diamondback moth to chlorantraniliprole was reduced from 1029 to 505. The results indicated that PxGST2L is partly responsible for chlorantraniliprole insecticide resistance in DBM. Our finding contributes to the understanding of the mechanism underlying resistance to chlorantraniliprole in the DBM, to develop effective resistance management tactics.

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