Identification and physiological function of CsPrip, a new aquaporin in Chilo suppressalis

文献类型： 外文期刊

第一作者： Lu, Ming-Xing

作者： Lu, Ming-Xing;He, Fu-Jing;Xu, Jing;Du, Yu-Zhou;Lu, Ming-Xing;He, Fu-Jing;Xu, Jing;Du, Yu-Zhou;Lu, Ming-Xing;Du, Yu-Zhou;Liu, Yang;Wang, Gui-Rong

作者机构：

关键词： Pyrocoelia rufa integral protein (Prip); Chilo suppressalis; Gene expression; Functional assay; Physiology

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 184 卷

页码：

收录情况： SCI

摘要： Aquaporin (AQP) transport solutes across cell membranes in both unicellular and multicellular organisms. In this study, the aquaporin CsPrip was identified in Chilo suppressalis, an important pest of rice. CsPrip was comprised of two variants, CsPrip_v1 and CsPrip_v2; the former variant was <103 bp was shorter than the latter, although both exhibited the same open reading frame (ORF). Transmembrane topology and protein structure analyses showed that CsPrip retained the conserved features of water-selective insect AQPs, including six transmembrane domains, two conserved hydrophobic asparagine-proline-alanine motifs and the aromatic/arginine constriction region. Expression in Xenopus oocytes revealed that CsPrip preferentially transported water and urea instead of trehalose and glycerol. The CsPrip transcript was expressed in multiple organs and tissues of C. suppressalis larvae and was most abundant in the hindgut and Malpighian tubules. CsPrip transcription was highest in male adults and was relatively stable throughout development. CsPrip expression in larvae was significantly altered by thermal stress, and relative humidity levels impacted CsPrip transcription in 3rd and 5th instar larvae. This study confirms that the aquaporin CsPrip performs multiple critical functions in maintaining water equilibrium in C. suppressalis.

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