A classic screening marker does not affect antennal electrophysiology but strongly regulates reproductive behaviours in Bactrocera dorsalis

文献类型： 外文期刊

第一作者： Wuyun, Qiqige

作者： Wuyun, Qiqige;Zhang, Yan;Yuan, Jinxi;Zhang, Jie;Ren, Cong;Wang, Qi;Yan, Shanchun;Liu, Wei;Wang, Guirong;Wuyun, Qiqige;Zhang, Yan;Yuan, Jinxi;Zhang, Jie;Ren, Cong;Wang, Qi;Yan, Shanchun;Liu, Wei;Wang, Guirong

作者机构：

关键词： Bactrocera dorsalis; Bdwhite; environmentally friendly pesticides; molecular targets; screening marker gene

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：2.6； 五年影响因子：2.9 ）

ISSN： 0962-1075

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The key phenotype white eye (white) has been used for decades to selectively remove females before release in sterile insect technique programs and as an effective screening marker in genetic engineering. Bactrocera dorsalis is a representative tephritid pest causing damage to more than 150 fruit crops. Yet, the function of white in important biological processes remains unclear in B. dorsalis. In this study, the impacts of the white gene on electrophysiology and reproductive behaviour in B. dorsalis were tested. The results indicated that knocking out Bdwhite disrupted eye pigmentation in adults, consistent with previous reports. Bdwhite did not affect the antennal electrophysiology response to 63 chemical components with various structures. However, reproductive behaviours in both males and females were significantly reduced in Bdwhite(-/-). Both pre-copulatory and copulation behaviours were significantly reduced in Bdwhite(-/-), and the effect was male-specific. Mutant females significantly delayed their oviposition towards gamma-octalactone, and the peak of oviposition behaviour towards orange juice was lost. These results show that Bdwhite might not be an ideal screening marker in functional gene research aiming to identify molecular targets for behaviour-modifying chemicals. Instead, owing to its strong effect on B. dorsalis sexual behaviours, the downstream genes regulated by Bdwhite or the genes from white-linked areas could be alternate molecular targets that promote the development of better behavioural modifying chemical-based pest management techniques.

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