Patulin Detoxification by Recombinant Manganese Peroxidase from Moniliophthora roreri Expressed by Pichia pastoris

文献类型： 外文期刊

第一作者： Wang, Shuai

作者： Wang, Shuai;Zhang, Yuhong;Liu, Bo;Zhang, Wei;Wang, Xiaolu;Luo, Huiying;Yao, Bin;Su, Xiaoyun;Penttinen, Leena;Hakulinen, Nina

作者机构：

关键词： patulin; mycotoxin; manganese peroxidase; apple juice; detoxification

期刊名称：TOXINS （ 影响因子：5.075； 五年影响因子：5.305 ）

ISSN：

年卷期： 2022 年 14 卷 7 期

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收录情况： SCI

摘要： The fungal secondary metabolite patulin is a mycotoxin widespread in foods and beverages which poses a serious threat to human health. However, no enzyme was known to be able to degrade this mycotoxin. For the first time, we discovered that a manganese peroxidase (MrMnP) from Moniliophthora roreri can efficiently degrade patulin. The MrMnP gene was cloned into pPICZ alpha(A) and then the recombinant plasmid was transformed into Pichia pastoris X-33. The recombinant strain produced extracellular manganese peroxidase with an activity of up to 3659.5 U/L. The manganese peroxidase MrMnP was able to rapidly degrade patulin, with hydroascladiol appearing as a main degradation product. Five mg/L of pure patulin were completely degraded within 5 h. Moreover, up to 95% of the toxin was eliminated in a simulated patulin-contaminated apple juice after 24 h. Using Escherichia coli as a model, it was demonstrated that the deconstruction of patulin led to detoxification. Collectively, these traits make MrMnP an intriguing candidate useful in enzymatic detoxification of patulin in foods and beverages.

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