Transcriptional and ultrastructural changes of macrophages after african swine fever virus infection

文献类型： 外文期刊

第一作者： Yuan, Cong

作者： Yuan, Cong;Duan, Yueyue;Li, Xiangtong;Zhang, Yu;Cao, Liyan;Feng, Tao;Wang, Qi;Zheng, Haixue;Yuan, Cong;Ge, Junwei;Yuan, Cong;Duan, Yueyue;Li, Xiangtong;Zhang, Yu;Cao, Liyan;Wang, Qi;Li, Xiangtong;Zhang, Yu;Zheng, Haixue

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关键词： ASFV; RNA-seq; Ultrastructure; Lysosome

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）

ISSN： 0378-1135

年卷期： 2024 年 293 卷

页码：

收录情况： SCI

摘要： African swine fever (ASF) is a highly impactful infectious disease in the swine industry, leading to substantial economic losses globally. The causative agent, African swine fever virus (ASFV), possesses intricate pathogenesis, warranting further exploration. In this study, we investigated the impact of ASFV infection on host gene transcription and organelle changes through macrophage transcriptome sequencing and ultrastructural transmission electron microscopy observation. According to the results of the transcriptome sequencing, ASFV infection led to significant alterations in the gene expression pattern of porcine bone marrow derived macrophages (BMDMs), with 2404 genes showing upregulation and 1579 genes downregulation. Cytokines, and chemokines were significant changes in the expression of BMDMs; there was significant activation of pattern recognition receptors such as Toll-like receptors and Nod-like receptors. According to the observation of the ultrastructure, mitochondrial damage and mitochondrial autophagy were widely present in ASFV-infected cells. The reduced number of macrophage pseudopodia suggested that virus-induced structural changes may compromise pathogen recognition, phagocytosis, and signal communication in macrophages. Additionally, the decreased size and inhibited acidification of secondary lysosomes in macrophages implied suppressed phagocytosis. Overall, ASFV infection resulted in significant changes in the expression of cytokines and chemokines, accompanied by the activation of NLR and TLR signaling pathways. We reported for the first time that ASFV infection led to a reduction in pseudopodia numbers and a decrease in the size and acidification of secondary lysosomes.

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