Genome Sequence of the Asian Honeybee in Pakistan Sheds Light on Its Phylogenetic Relationship with Other Honeybees

文献类型： 外文期刊

第一作者： Tan, Hongwei

作者： Tan, Hongwei;Zhang, Ze;Tan, Hongwei;Naeem, Muhammad;Ali, Hussain;Shakeel, Muhammad;Kuang, Haiou;Sun, Cheng

作者机构：

关键词： Apis cerana; genome sequencing; phylogeny

期刊名称：INSECTS （ 影响因子：2.769； 五年影响因子：3.046 ）

ISSN：

年卷期： 2021 年 12 卷 7 期

页码：

收录情况： SCI

摘要： Simple Summary The Asian honeybee, Apis cerana, is used for honey production and pollination services in Pakistan. However, its genome sequence is still unknown. We collected A. cerana samples from its main rearing region in Pakistan and performed whole genome sequencing. We obtained a remarkably complete genome sequence for A. cerana in Pakistan, from which we identified a total of 11,864 protein-coding genes. Phylogeny analysis indicated an unexpectedly close relationship between A. cerana in Pakistan and those in China, suggesting a potential human introduction of the species between the two countries. Our results will facilitate the genetic improvement and conservation of A. cerana in Pakistan. In Pakistan, Apis cerana, the Asian honeybee, has been used for honey production and pollination services. However, its genomic makeup and phylogenetic relationship with those in other countries are still unknown. We collected A. cerana samples from the main cerana-keeping region in Pakistan and performed whole genome sequencing. A total of 28 Gb of Illumina shotgun reads were generated, which were used to assemble the genome. The obtained genome assembly had a total length of 214 Mb, with a GC content of 32.77%. The assembly had a scaffold N50 of 2.85 Mb and a BUSCO completeness score of 99%, suggesting a remarkably complete genome sequence for A. cerana in Pakistan. A MAKER pipeline was employed to annotate the genome sequence, and a total of 11,864 protein-coding genes were identified. Of them, 6750 genes were assigned at least one GO term, and 8813 genes were annotated with at least one protein domain. Genome-scale phylogeny analysis indicated an unexpectedly close relationship between A. cerana in Pakistan and those in China, suggesting a potential human introduction of the species between the two countries. Our results will facilitate the genetic improvement and conservation of A. cerana in Pakistan.

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