Lentinan PLGA-stabilized pickering emulsion for the enhanced vaccination

文献类型： 外文期刊

第一作者： Jiao, Lina

作者： Jiao, Lina;Liu, Zhenguang;Zhang, Yue;Feng, Zian;Gu, Pengfei;Liu, Jiaguo;Wu, Yi;Wang, Deyun;Jiao, Lina;Liu, Zhenguang;Zhang, Yue;Feng, Zian;Gu, Pengfei;Liu, Jiaguo;Wu, Yi;Wang, Deyun;Huang, Yee

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关键词： Adjuvant; Ovalbumin; Vaccine delivery; Immune responses; Lymphocyte proliferation

期刊名称：INTERNATIONAL JOURNAL OF PHARMACEUTICS （ 影响因子：6.51； 五年影响因子：6.274 ）

ISSN： 0378-5173

年卷期： 2022 年 611 卷

页码：

收录情况： SCI

摘要： Lentinan (LNT), a beta-1,3-linked-d-glucan with beta-1,6 glucose branches, is the main bioactive component extracted from Lentinus edodes. As a carbohydrate polymer, it has attracted increasingly attention because of immune enhancement effect. Pickering emulsion has been widely used in biomedicine due to its great stability, high loading capacity, and appreciable biocompatibility. The aim of this study is to construct an adjuvant delivery system (LNTPP/OVA) (Lentinan PLGA-stabilized Pickering emulsion loading OVA antigen) which can enhance the immune activity of LNT and can together deliver model protein antigen ovalbumin (OVA) into the organism. The characterization of the LNTPP/OVA was demonstrated that the size of LNTPP/OVA was around 1050.68 nm and was stable to store at least 28 days. Pickering emulsion was spherical shape like the raspberry with the high antigen load rate at around 82.53%. Moreover, the adjuvant effect of LNTPP/OVA formulation was detected. Compared with LNT/OVA formulation, our experimental results showed that LNTPP/OVA could promote the uptake of the OVA-antigen by macrophages in vitro. In vivo experiments, LNTPP/OVA facilitated the activation of dendritic cells (DCs) and induced strong humoral and cellular immune responses carrying a Th1 and Th2 immune responses. Therefore, LNTPP/OVA formulation have the latent capacity as a vaccine transmission system.

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