Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice

文献类型： 外文期刊

第一作者： Teng, Fei

作者： Teng, Fei;Xiong, Xiaoyu;Zhang, Songsong;Wang, Xiaona;Zhou, Han;Li, Jiaxuan;Li, Yijing;Jiang, Yanping;Cui, Wen;Tang, Lijie;Wang, Li;Qiao, Xinyuan;Li, Guiwei;Wang, Ruichong;Zhang, Lanlan

作者机构：

关键词： Staphylococcus aureus; mastitis; drug-resistant; phage

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 3 期

页码：

收录情况： SCI

摘要： The primary aim of this study was to evaluate the efficacy of phage against mastitis induced by drug-resistant S. aureus in a mouse model. In this study, five S. aureus phages-4086-1, 4086-2, 4086-3, 4086-4, and 4086-6-were isolated from milk samples secreted by mastitis cows. Transmission electron microscopy showed that all the five phages had icosahedral heads and short non-contractile tails, which are typical characteristics of the family Podoviridae. All these phages were species-specific against S. aureus. The one-step growth curve showed a short latency period (10-20 min) and high burst size (up to 400 PFU/infected cell). To evaluate the effectiveness of the phage 4086-1 in the treatment against mastitis, a mouse model of mastitis was challenged with drug-resistant S. aureus. The results showed the proliferation of S. aureus in the mammary glands was significantly inhibited after treating by phage 4086-1. The concentrations of TNF-alpha and IL-6 decreased significantly, which demonstrated the phages could effectively alleviate the inflammatory responses. Furthermore, the histopathological analysis showed that inflammatory infiltration in the mammary glands was significantly reduced. These results demonstrate that phage may be a promising alternative therapy against mastitis caused by drug-resistant S. aureus.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序