PE (Prickly Eggplant) encoding a cytokinin-activating enzyme responsible for the formation of prickles in eggplant

文献类型： 外文期刊

第一作者： Zhang, Lei

作者： Zhang, Lei;Zhang, Runzhi;Yan, Ping;Zeng, Liqian;Zhao, Weiwei;Feng, Huiqian;Hou, Wenqian;Mu, Ruyu

作者机构： Jiangsu Normal Univ, Jiangsu Int Joint Ctr Genom, Sch Life Sci, Jiangsu Key Lab Phylogen & Comparat Genom, Xuzhou 221100, Jiangsu, Peoples R China;Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 2023影响因子：7.6； 五年影响因子：8.2 ）

ISSN： 2662-6810

年卷期： 2024 年 11 卷 7 期

页码：

收录情况： SCI

摘要： Eggplant is one of the most important vegetables worldwide, with some varieties displaying prickles. These prickles, present on the leaves, stems, and fruit calyxes, posing challenges during cultivation, harvesting, and transportation, making them an undesirable agronomic trait. However, the genetic mechanisms underlying prickle morphogenesis in eggplant remain poorly understood, impeding genetic improvements. In this study, genetic analyses revealed that prickle morphogenesis is governed by a single dominant nuclear gene, termed PE (Prickly Eggplant). Subsequent bulk segregant RNA-sequencing (BSR-seq) and linkage analysis preliminarily mapped PE to chromosome 6. This locus was then fine mapped to a 9233 bp interval in a segregating population of 1109 plants, harboring only one candidate gene, SmLOG1, which encodes a LONELY GUY (LOG)-family cytokinin biosynthetic enzyme. Expression analyses via transcriptome and qRT-PCR demonstrate that SmLOG1 is predominantly expressed in immature prickles. CRISPR-Cas9 knockout experiments targeting SmLOG1 in prickly parental line 'PI 381159' abolished prickles across all tissues, confirming its critical role in prickle morphogenesis. Sequence analysis of SmLOG1 pinpointed variations solely within the non-coding region. We developed a cleaved amplified polymorphic sequences (CAPS) marker from a distinct SNP located at -735-bp within the SmLOG1 promoter, finding significant association with prickle variation in 190 eggplant germplasms. These findings enhance our understanding of the molecular mechanisms governing prickle development in eggplant and facilitate the use of marker-assisted selection (MAS) for breeding prickleless cultivars.

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