Protective effects of Fagopyrum dibotrys on oxidized oil-induced oxidative stress, intestinal barrier impairment, and altered cecal microbiota in broiler chickens

文献类型： 外文期刊

第一作者： Chen, Zhaojun

作者： Chen, Zhaojun;Dai, Guotao;Wu, Xian;Li, Lina;Tian, Yujie;Tan, Lulin;Chen, Zhaojun;Chen, Zhaojun

作者机构：

关键词： broiler; F; dibotrys; oxidized oil; antioxidant status; cecal microbiota

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 4 期

页码：

收录情况： SCI

摘要： The objective of this study was to evalu-ate protective effects of Fagopyrum dibotrys on antioxi-dant ability, intestinal barrier functions, and cecal microbiota in broiler chickens fed oxidized soybean oil. A total of 640 male Tiejiaoma broilers were randomly assigned to 8 treatments with 8 cages (10 birds per cage), as follows: birds fed basal diets containing fresh soybean oil and 0, 0.5, 1, or 2% F. dibotrys (FSCON, FSFAL, FSFAM, and FSFAH, respectively), and birds fed basal diets containing oxidized oil and 0, 0.5, 1, or 2% F. dibotrys (OSCON, OSFAL, OSFAM, and OSFAH). Oxidized oil significantly decreased transcrip-tion of Nrf2 and its downstream genes, including CAT and SOD1 in the jejunal mucosa, increased jejunal mucosa IL-6 mRNA expression, and decreased jejunal mucosa IL-22 mRNA expression and downregulated Claudin-1 and ZO-1; however, all these effects were reversed by F. dibotrys. Either 1 or 2% F. dibotrys allevi-ated the decreased liver SOD induced by oxidized oil on d 42. The decreased SOD and GPX, and increased MDA induced by oxidized oil were reversed by adding 1 or 2% F. dibotrys in jejunal mucosa. In addition, based on 16S rDNA, 2% F. dibotrys promoted the Firmicutes phylum and Candidatus_Arthromitus genera, but suppressed the Proteobacteria phylum and Streptococcus, Entero-coccus, and Escherichia genera. In summary, oxidative stress induced by oxidized oil was ameliorated by F. dibotrys upregulating transcription of Nrf2 and its downstream genes to restore redox balance, reinforcing the intestinal barrier via higher expression of Claudin-1/ ZO-1, ameliorating the inflammatory response by regu-lating expression of IL-6 and IL-22, and facilitating growth of Candidatus_arthromitus in the cecum. There-fore, F. dibotrys has potential as a feed additive for poul-try by ameliorating oxidative stress caused by oxidized oil, enhancing barrier function, and improving gut microbiome composition.

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