Proteomic, transcriptomic, biochemical, and physio-anatomical analyses provide insights into energy metabolism, light usage, and photosynthesis in cigar tobacco under different light intensities

文献类型： 外文期刊

第一作者： Ma, Xinghua

作者： Ma, Xinghua;Ren, Xiaochun;Wu, Xiaoying;Chen, Keling;Khan, Rayyan;Gao, Huajun

作者机构：

关键词： Cigar Tobacco; Low light; Light intensities; Shading; Transcriptome and Proteome

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.9； 五年影响因子：6.0 ）

ISSN： 0926-6690

年卷期： 2023 年 198 卷

页码：

收录情况： SCI

摘要： Low light (LL) resulting in shading are among the detrimental abiotic stresses limiting plant growth and suppressing crop productivity. Shading is a key cultivation technique in cigar wrapper tobacco production but little is known about its impact on transcriptional and translational regulatory networks. Here, we integrate transcriptomic and proteomic profiling with physio-biochemical and anatomical analyses under different light intensities [T200 (200 mu mol m- 2 s- 1), T100 (100 mu mol m- 2 s- 1), and T50 (50 mu mol m- 2 s- 1)] to uncover the underlying molecular response mechanisms of tobacco plants. We found that the leaf anatomical structure impacts photosynthetic capacity and that LL intensities (particularly T50) decrease leaf and palisade thickness (42%) and spongy tissues (16%), which leads to a lower photosynthetic rate (84%) compared with T200. Furthermore, we identified 3045 and 590 significantly differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in the transcriptome and proteome of cigar tobacco, respectively. A total of 110 pairs were correlated which were upregulated in photosynthesis-antenna proteins, photosynthesis, and defense/ detoxification-related pathways, according to integrated omics analyses, and downregulated in tyrosine metabolism, starch and sucrose metabolism, mitochondrial electron transport chain, and glycolysis pathways; and associated with decreased activities of glyceraldehyde-3-phosphate dehydrogenase (31%), starch phosphorylase (25%), and pyruvate kinase (24%) enzymes related to glycolysis. Our results show that cigar tobacco efficiently utilizes low light to reconfigure its energy metabolism, and offer profound insights into the response mechanisms at the physio-biochemical, anatomical, and molecular levels. This study thus represents a valuable resource of genes and proteins for future functional studies underlying LL response.

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