Identification of bta-miR-15a similar to 16a cluster expression, localization and regulated target in Holsteins

文献类型： 外文期刊

第一作者： Ju, Zhihua

作者： Ju, Zhihua;Zhao, Shuhong;Ju, Zhihua;Huang, Jinming;Jiang, Qiang;Wang, Changfa;Wang, Xiuge

作者机构：

关键词： bta-miR-15a similar to 16a cluster; Expression and localization; Regulated target; Mastitis; Holstein

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）

ISSN： 0890-8508

年卷期： 2018 年 40 卷

页码：

收录情况： SCI

摘要： Bovine mastitis is an inflammation response of the mammary gland tissues caused mainly by pathogenic bacteria in cows. Previous studies showed that bta-miR-15a and bta-miR-16a modulate immunity and inflammation responses. In this study, we investigated the expression pattern and tissue localization of bta-miR-15a and bta-miR-16a. The expression levels of bta-miR-15a and bta-miR-16a were significantly upregulated in mammary tissues and blood neutrophils of mastitis-infected cows, compared with those of healthy cows (P < 0.05). Through in situ hybridization, we examined the tissue localization of bta-miR-15a and bta-miR-16a and found that they were expressed in the ductal and acinar cells of mammary gland tissues, where they had a stronger expression signal in the mammary tissues of cows with mastitis than that in healthy cows' tissues. Moreover, we identified CD163 as the target gene of bta-miR-15a and bta-miR-16a. Luciferase assay indicated that bta-miR-15a, bta-miR-16a, and bta-miR-15a similar to 16a cluster led to the significant reduction in the luciferase activity of CD163 3'UTR vector (P < 0.05). Meanwhile, the luciferase activity had a significantly low value compared with that of single bta-miR-15a or bta-miR-16a plasmid (P < 0.05) in the presence of bta-miR-15a similar to 16a cluster. The bta-miR-15a similar to 16a cluster may function more effectively in inhibiting luciferase reporter gene activity of target gene CD163 than single miRNA. Our study provides an insight into the relationship between bovine mastitis and gene expression of bta-miR-15a/16a, which suggested that bta-miR-15a similar to 16a cluster may play a role against mastitis by binding to target CD163 gene in Holstein dairy cattle.

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[1]Identification of bta-miR-15a similar to 16a cluster expression, localization and regulated target in Holsteins. Ju, Zhihua,Zhao, Shuhong,Ju, Zhihua,Huang, Jinming,Jiang, Qiang,Wang, Changfa,Wang, Xiuge. 2018

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