Comparison between Lactobacillus rhamnosus GG and LuxS-deficient strain in regulating gut barrier function and inflammation in early-weaned piglets

文献类型： 外文期刊

第一作者： Deng, Zhaoxi

作者： Deng, Zhaoxi;Hua, Weidong;Deng, Zhaoxi;Dai, Jinyan;Wei, Yusen;Ma, Yanfei;Mao, Yingying;Zhang, Jinzhi;Wang, Haifeng;Deng, Zhaoxi

作者机构： Zhejiang Acad Agr Sci, Expt Livestock Farm Anim Husb & Vet Res Inst, Hangzhou, Peoples R China;Zhejiang Univ, Coll Anim Sci, Key Lab Mol Anim Nutr, Minist Educ MOE, Hangzhou, Peoples R China;Sichuan Univ, Lab Anim Ctr, Chengdu, Peoples R China

关键词： Lactobacillus rhamnosus GG; luxS mutant; quorum sensing; early-weaning; piglets; intestinal barrier function

期刊名称：FRONTIERS IN IMMUNOLOGY （ 2021影响因子：8.786； 五年影响因子：8.876 ）

ISSN： 1664-3224

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： BackgroundEarly weaning-induced stress impairs the intestinal barrier function and adversely affects the health of piglet. Probiotics can be used to prevent and treat various intestinal diseases. Lactobacillus rhamnosus GG (LGG) has an LuxS/AI-2 quorum sensing (QS) system that senses environmental changes through chemical signaling molecules. The aim of the study was to explore whether luxS mutant affects the protective role of LGG in the gut barrier of weaned piglets by comparing the luxS mutant (Delta luxS) with its wild-type (WT). MethodsNewborn piglets were orally administered with WT and Delta luxS at dosage of 10(9) CFU, respectively. Accordingly, newborn piglets in the Con group were orally administered with PBS. Piglets were weaned on day 21 and euthanized on day 24, three days following weaning. ResultsSupplementation of Delta luxS in advance significantly boosted the relative abundances of healthy microbes (including Catenibacterium, Eubacterium, Lachnospiraceae and Bifidobacterium). WT and Delta luxS maintain intestinal barrier function mainly by promoting intestinal villus to crypt ratio (VCR), occludin protein expression and mucus secretion (P<0.05). Furthermore, LGG reduces pro-inflammatory mediators by inhibiting TLR4 and MAPK signal transduction (P<0.05). ConclusionBoth WT and Delta luxS were shown to resist weaning stress by enhancing the intestinal barrier function of piglets. It has to be said that the ability of Delta luxS to maintain intestinal tissue morphology and promote mucus secretion significantly decreased compared with that of WT.

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