Combination of ultrasound and chlorogenic acid for inactivation of planktonic and biofilm cells of Pseudomonas fluorescens

文献类型： 外文期刊

第一作者： Sun, Jinyue

作者： Sun, Jinyue;Sun, Zhilan;Wang, Debao;Liu, Fang;Wang, Daoying;Sun, Jinyue;Sun, Zhilan;Wang, Debao;Liu, Fang;Wang, Daoying;Huang, Lihan;Du, Lihui

作者机构：

关键词： Ultrasound; Chlorogenic acid; Pseudomonas fluorescens; Biofilm

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：7.425； 五年影响因子：7.716 ）

ISSN： 0963-9969

年卷期： 2022 年 155 卷

页码：

收录情况： SCI

摘要： This study investigated the synergistic efficacy of ultrasound (US, 50 kHz, 400 W) in combination with chlorogenic acid (CA; 0, 0.5, 1, and 2%) to inactivate Pseudomonas fluorescens planktonic and biofilm cells. The P. fluorescens planktonic and biofilm cells were treated with CA with and without US for 5, 10, 20, 30, or 60 min. Results showed that US enhanced the efficacy of CA for inactivation of both P. fluorescens planktonic and biofilm cells. Treatment with 2% CA and US could completely inactivate P. fluorescens planktonic cells within 10 min and the biofilm cells within 30 min. Confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), ATP, and nucleic acid release assays indicated that the combination of US and CA could quickly damage the integrity of P. fluorescens planktonic and biofilm cell membranes. The combined treatment effectively inactivates P. fluorescens planktonic and biofilm cells via the synergetic destruction of the biofilm structure and bacterial cell integrity.

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