Promoter Variation of the Key Apple Fruit Texture Related Gene MdPG1 and the Upstream Regulation Analysis

文献类型： 外文期刊

第一作者： Wu, Mengmeng

作者： Wu, Mengmeng;Cao, Shangyin;Wu, Mengmeng;Luo, Zhengrong

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关键词： MdPG1; Malus x domestica; MdCBF2; lncRNA(PG1); cis-regulation

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

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年卷期： 2023 年 12 卷 7 期

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收录情况： SCI

摘要： MdPG1 encoding polygalacturonase in apple (Malus x domestica) is a key gene associated with fruit firmness and texture variations among apple cultivars. However, the causative variants of MdPG1 are still not known. In this study, we identified a SNPA/C variant within an ERF-binding element located in the promoter region of MdPG1. The promoter containing the ERF-binding element with SNPA, rather than the SNPC, could be strongly bound and activated by MdCBF2, a member of the AP2/ERF transcription factor family, as determined by yeast-one-hybrid and dual-luciferase reporter assays. We also demonstrated that the presence of a novel long non-coding RNA, lncRNA(PG1), in the promoter of MdPG1 was a causative variant. lncRNA(PG1) was specifically expressed in fruit tissues postharvest. lncRNA(PG1) could reduce promoter activity when it was fused to the promoter of MdPG1 and a tobacco gene encoding Mg-chelatase H subunit (NtCHLH) in transgenic tobacco cells but could not reduce promoter activity when it was supplied in a separate gene construct, indicating a cis-regulatory effect. Our results provide new insights into genetic regulation of MdPG1 allele expression and are also useful for the development of elite apple cultivars.

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