Identification, expression and molecular polymorphism of T-cell receptors α and β from the glacial relict Hucho bleekeri

文献类型： 外文期刊

第一作者： Lin, Jue

作者： Lin, Jue;Wu, Xiaoyun;Liu, Zhao;Yang, Huanchao;Chen, Yanling;Li, Hua;Yu, Yi;Tu, Quanyu;Chen, Yeyu;Lin, Jue;Wu, Xiaoyun;Liu, Zhao;Yang, Huanchao;Chen, Yanling;Li, Hua;Yu, Yi;Tu, Quanyu;Chen, Yeyu

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关键词： T -cell receptor; Hucho bleekeri; Immune response; Molecular polymorphism

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2024 年 148 卷

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收录情况： SCI

摘要： The T-cell receptor (TCR) is a specific molecule on the surface of all T cells that mediates cellular adaptive immune responses to antigens. Hucho bleekeri is a critically endangered species and is regarded as a glacial relict that has the lowest-latitude distribution compared with any Eurasian salmonid. In the present study, two TCR genes, namely, TCR alpha and fi, were identified and characterized in H. bleekeri. Both TCR alpha and TCR fi have typical TCR structures, including the IgV domain, IgC domain, connecting peptide, transmembrane and cytoplasmic domains. The two TCR genes were constitutionally expressed in various tissues, with the highest expression found in the spleen for TCR alpha and in the trunk kidney for TCR fi. Challenge of H. bleekeri with LPS or poly(I:C) resulted in significant upregulation of both TCR alpha and fi expression in headkidney and spleen primary cells, indicating their potential roles in the immune response. Molecular polymorphism analysis of the whole ORF regions of TCR alpha and fi in different individuals revealed high diversity of IgV domains of these two genes, especially in complementarity-determining region (CDR) 3. The ratio of nonsynonymous substitution occurred at a significantly higher frequency than synonymous substitution in the CDR of TCR alpha and fi, demonstrating the existence of positive selection. The results obtained in the present study enhance our understanding of TCR roles in regulating immune mechanisms and provide new information for the study of TCR lineage diversity in fish.

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