Identification and functional prediction of CircRNAs of developing seeds in high oleic acid sunflower (Helianthus annuus L.)

文献类型： 外文期刊

第一作者： Zhou, Fei

作者： Zhou, Fei;Huang, Xutang;Zhou, Fei;Liu, Yan;Wang, Wenjun;Wu, Liren;Ma, Jun;Zhang, Shuquan;Wang, Jing;Yuan, Hongmei;Huang, Xutang;Feng, Fujuan

作者机构：

关键词： Sunflower; CircRNAs; Host genes; Seed development; Oil biosynthesis; High oleic acid

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.736； 五年影响因子：2.983 ）

ISSN： 0137-5881

年卷期： 2023 年 45 卷 1 期

页码：

收录情况： SCI

摘要： With the rapid developments of bioinformatics technologies, various kinds of circRNAs in many species have been developed and the according pivotal roles were also revealed. However, a few studies describing circRNAs involved in seed development and oil biosynthesis processes have been reported, with no studies reported in sunflower (Helianthus annuus L.) to date. In this current research, using a kind of sunflower seed with its high oleic acid content, we first measured seed fresh weight, dry weight, and moisture content during different seed developmental stages. Next, high-throughput sequencing and bioinformatics methodologies were employed to detect circRNAs in seed specimens collected during three representative developmental stages. Ultimately, a total of 3482 new circRNAs were detected, of which 2723, 2910, and 1538 were found to be differentially expressed (DE) between paired time points of L7 vs. L22DAF, L7 vs. L37DAF, and L22 vs. L37DAF, respectively. Gene ontology (GO) analysis assigned lipid metabolism-associated GO functional terms to some host genes of DEcircRNAs. Notably, both KEGG functional pathway enrichment analysis results and GO results suggested that host DEcircRNA gene functions were correlated with pathways that in relation to fatty acid metabolism (FAM). Finally, networks comprised of DEcircRNAs and host genes with FAM pathway-associated functions were constructed. Results of network analysis highlighted important functional associations between DEcircRNAs and several key enzymes, including delta-12 desaturase (FAD2). These results revealed potential functional roles of circRNAs in sunflower, including regulatory roles in seed development and oil biosynthesis processes. This work also describes an effective strategy for investigating novel circRNAs in sunflower.

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