Characterization of phosphofructokinase (PFK) from mud crab Scylla paramamosain and its role in mud crab dicistrovirus-1 proliferation

文献类型： 外文期刊

第一作者： Jie, Yu-Kun

作者： Jie, Yu-Kun;Xie, Jia-Wei;Cheng, Chang-Hong;Ma, Hong-Ling;Liu, Guang-Xin;Jiang, Jian-Jun;Deng, Yi-Qin;Feng, Juan;Guo, Zhi-Xun;Jie, Yu-Kun;Xie, Jia-Wei;Luo, Zhi-Ping

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关键词： Phosphofructokinase; Scylla paramamosain; Glycolysis; MCDV-1

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2022 年 124 卷

页码：

收录情况： SCI

摘要： Phosphofructokinase (PFK), the key enzyme of glycolysis, can catalyze the irreversible transphosphorylation of fructose-6-phosphate forming fructose-1, 6-biphosphate. In the present study, a PFK gene from the mud crab Scylla paramamosain, named SpPFK, was cloned and characterized. The full length of SpPFK contained a 5 ' untranslated region (UTR) of 249 bp, an open reading frame of 2,859 bp, and a 3 ' UTR of 1,248 bp. The mRNA of SpPFK was highly expressed in the gill, followed by the hemocytes and muscle. The expression of SpPFK was significantly up-regulated after mud crab dicistrovirus-1 (MCDV-1) infection. Knocking down SpPFK in vivo by RNA interference significantly reduced the expression of lactate dehydrogenase after MCDV-1 infection. Furthermore, silencing of SpPFK in vivo increased the survival rate of mud crabs and decreased the MCDV-1 copies in the gill and hepatopancreas after MCDV-1 infection. All these results suggested that SpPFK could play an important role in the process of MCDV-1 proliferation in mud crab.

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