The Sodium/Calcium Exchanger PcNCX1-Mediated Ca2+ Efflux Is Involved in Cinnamaldehyde-Induced Cell-Wall Defects of Phytophthora capsici

文献类型： 外文期刊

第一作者： Qi, Zhongqiang

作者： Qi, Zhongqiang;Li, Lina;Xu, Cunfa;Liu, Muxing;Wang, Yousheng;Zhang, Li;Chen, Jian;Lu, Haiyan;Shi, Zhiqi

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关键词： calcium; cell wall integrity; cinnamaldehyde; PcNCX1; Phytophthora capsici

期刊名称：AGRONOMY-BASEL （ 影响因子：3.949； 五年影响因子：4.117 ）

ISSN：

年卷期： 2022 年 12 卷 8 期

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收录情况： SCI

摘要： Phytophthora capsici is one of the devastating pathogens, causing foliar blight, root rot, and fruit rot in peppers. Cinnamaldehyde (CA) is a natural compound coming from Cinnamomum cassia. The medicinal properties of CA have been widely identified. Limited knowledge is known about the application of CA in agriculture. In this study, CA significantly inhibited P. capsici, which further suppressed Phytophthora blights in both pepper seedlings and pepper fruits. Treatment with CA resulted in collapsed and fragmented hyphae, accompanying the increase in MDA (malondialdehyde) content and the decrease in intercellular glycerol content in hyphae. CA also inhibited the growth of wild type yeast. The yeast mutant Delta Yvc1 with a deletion of Yvc1 (a Ca2+ transporter) showed decreased sensitivity to CA. The transformation of PcNCX1, a sodium/calcium exchanger from P. capsici, into Delta Yvc1 restored its sensitivity to CA. The transformant carrying PcNCX1 also showed restored Ca2+ efflux upon CA treatment. RNA-seq analysis showed that CA treatments resulted in the down-regulation of a set of genes encoding for calcium-related proteins. Collectively, our study demonstrates that the antifungal activity of CA against P. capsici may be associated with PcNCX1-mediated Ca2+ efflux. Our results provide crucial insights into the antimicrobial action of CA.

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