Uterine inflammation status modulates eggshell mineralization via calcium transport and matrix protein synthesis in laying hens

文献类型： 外文期刊

第一作者： Feng, Jia

作者： Feng, Jia;Lu, Mingyuan;Ma, Lingling;Zhang, Haijun;Wu, Shugeng;Qiu, Kai;Qi, Guanghai;Wang, Jing;Feng, Jia;Min, Yuna

作者机构：

关键词： Uterine inflammation; Eggshell ultrastructure; Mechanical property; Laying hen; Lipopolysaccharide challenge; Essential oil

期刊名称：ANIMAL NUTRITION （ 影响因子：6.3； 五年影响因子：6.9 ）

ISSN： 2405-6383

年卷期： 2023 年 13 卷

页码：

收录情况： SCI

摘要： This study explored the effects of uterine inflammation on eggshell mineralization, ultrastructure and mechanical properties in laying hens modified by a lipopolysaccharide (LPS) challenge or dietary essential oil (EO) addition. In trial 1, a total of 72 Hy-line Brown layers at 36 wk of age were randomly assigned to 3 treatment groups (n = 8), where they were intravenously injected with phosphate buffered saline, LPS at 1 mg/kg body weight, or LPS 3 times at 24-h intervals. In trial 2, a total of 288 Hy-line Brown layers at 60 wk of age were randomly divided into 4 groups (n = 8), where they were fed basal diets supplemented with EO at 0, 50,100 and 200 mg/kg for 12 wk. A uterine inflammation model was constructed with LPS treatment, indicated by the elevated expression of IL-1b and TNF-a (P < 0.05) and lymphocyte infiltration. Uterine inflammation caused remarkable decreases in eggshell thickness and mechanical properties with structure deteriorations (P < 0.05). Uterine inflammation stimulated the expression of matrix proteins ovotransferrin (TF) and ovalbumin (OVAL), while depressing the mRNA levels of calbindin-1 (CALB1) and osteopontin in uterine mucosa (P < 0.05). In contrast, EO addition alleviated uterine inflammation, evidenced by depressed levels of IL-1b and IL-6 (P < 0.05). There was a significant elevation in shell thickness and breaking strength following EO intervention (P < 0.05), and these effects were maximized at addition of 100 mg/kg. Further, EO improved shell ultrastructure including more early fusion, less type B mammillae, and increased effective thickness (P < 0.05). The alleviated inflammation decreased the expression of OVAL and TF, whereas ion transport genes like CALB1 and solute carrier family 26 member 9 were upregulated (P < 0.05). Our findings suggest that inflam-matory status can impact uterine functions in calcium transport and the synthesis of matrix proteins especially such as OVAL and TF, which in turn modulates calcium precipitation and ultrastructure for-mation, thereby determining eggshell mechanical properties. These findings provide a novel insight into the uterine inflammation-mediated modifications of eggshell quality.(c) 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序