Interpretation of the Genotype by Tissue Interactions of Four Genes (AFP1, CIRP, YB-1, and HMGB1) in Takifugu rubripes Under Different Low-Temperature Conditions

文献类型： 外文期刊

第一作者： Wang, Xinan

作者： Wang, Xinan;Liu, Zhifeng;Ma, Aijun;Wang, Xinan;Liu, Zhifeng;Ma, Aijun

作者机构：

关键词： genotype; tissue; interactions; Takifugu rubripes; resistance genes to low temperature

期刊名称：FRONTIERS IN MOLECULAR BIOSCIENCES （ 影响因子：6.113； 五年影响因子：6.122 ）

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年卷期： 2022 年 9 卷

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收录情况： SCI

摘要： Background: The differential expression of the same gene in different tissues could be due to the genotype effect, tissue effect, and/or genotype x tissue interactions. However, the genetic mechanisms responsible for this differential expression have not been reported to date.Methods: Four resistance genes to low temperature, the genes for antifreeze protein (AFP), cold induced RNA binding protein (CIRP), high mobility group protein box-1 (HMGB1), and Y-box binding protein (YB-1), were measured by PCR in the liver, spleen, kidney, brain, heart, intestine, muscle, gonad, and skin of Takifugu rubripes cultured under different temperature conditions (18, 13, 8, and 5 degrees C). Split-split-plot analysis of variance, additive main effects and multiplicative interaction (AMMI) and genotype main effects and genotype x environment interaction (GGE) biplot analysis were used to evaluate the effects of genotype x tissue interactions on gene expression.Results: The results of split-split-plot analysis of variance showed that water temperature has a significant effect on the expression of T. rubripes cold resistant genes, while tissue x gene interaction has a highly significant effect on it. AMMI analysis showed that the contributions of genotype, tissue, and genotype x tissue interactions to the total variation in gene expression followed two trends: 1) as temperature decreased, the gene effect increased gradually and the genotype x tissue interaction decreased gradually; 2) the gene effect at 18 and 13 degrees C was significantly lower than that at 8 and 5 degrees C, while the interaction at 18 and 13 degrees C was significantly higher than that at 8 and 5 degrees C. GGE analysis showed that: at all temperatures except 8 degrees C, the expression rankings of all four genes were highly positively correlated in all tissues except muscle; the expression stability of the genes was the same at 18 degrees C/13 degrees C and at 8 degrees C/5 degrees C; and AFP1 showed the best expression and stability among the four genes.Conclusion: 8 degrees C/5 degrees C as the suitable temperature for such experiments for T. rubripes. Among the four antifreeze genes evaluated in this study, AFP1 had the best expression and stability.

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