Antitoxin EndoAI can induce disease resistance in tobacco as a protein elicitor

文献类型： 外文期刊

第一作者： Liu, Wen Zhi

作者： Liu, Wen Zhi;Yu, Wen Qing;Xie, Hong Ping;Qiu, Zu Ming;Yan, Bo Wei;Yu, Wen Qing;Li, Peng;Yan, Feng Chao;Yan, Bo Wei;Zhao, Chang Jiang;Yan, Bo Wei;Yan, Bo Wei;Wang, Gui Jiang;Zhang, Li Guo

作者机构：

关键词： Antitoxin; Elicitor; Hypersensitive response; Reactive oxygen species; Induced systemic resistance; Pathogenesis-related gene

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：4.839； 五年影响因子：5.83 ）

ISSN：

年卷期： 2021 年 8 卷 1 期

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收录情况： SCI

摘要： Background: The antitoxin EndoAI is a TA system component that directly inhibits EndoA activity in vitro. The targeted activation of a TA system represents a potentially novel antimicrobial or antiviral strategy. However, whether the antitoxin functions alone and can induce plant disease resistance remain unknown. Results: An endoAI was previously identified in the genome of Paenibacillus terrae NK3-4. It underwent a bioinformatics analysis, cloned and expressed in Escherichia coli. Then the functions of EndoAI inducing plant resistance to diseases as an elicitor were evaluated. The results showed that, EndoAI is a stable, alkaline, and hydrophilic protein, with a J-shaped three-dimensional structure in the absence of a ligand. It was clustered on the same branch with an antitoxin from Paenibacillus polymyxa SC2. Ectopically expressed EndoAI triggered a reactive oxygen species burst and a positive hypersensitive response (HR) in tobacco leaves. Moreover, 2 mu mol EndoAI induced HR activity in tomato leaf, and it remained active after a 15-min exposure at 4-50 degrees C, and pH 6-8. Additionally, EndoAI induced plant systemic resistance against Alternaria alternata and tobacco mosaic virus, and the up-regulated transcription of PR genes, including PR1a, PR1b, PR5, PDF1.2, COL1, NPR1, and PAL. Conclusions: These results imply that EndoAI may enhance the disease resistance of tobacco by promoting a series of early defense responses and up-regulating PR gene expression. These findings are relevant for future investigations on the mechanism underlying the EndoAI-plant interaction that leads to enhanced disease resistance. Furthermore, the endoAI may be useful for developing effective biocontrol agents to protect plants from diseases.

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