文献类型： 外文期刊

第一作者： Dan-Zhi, Zhang

作者： Dan-Zhi, Zhang;Li-Gan, Zhang;Dan-Zhi, Zhang;Qiang, Yin;Wen-Jing, Xu;Yong, Fang;Zheng-Tong, Jiao;Zuo-Jun, Liu;Shi-Chun, Ma

作者机构：

关键词： Anaerobic digestion; Strring; Organic acid metabolites; Microbial communities

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.193； 五年影响因子：0.987 ）

ISSN： 0253-3820

年卷期： 2022 年 50 卷 8 期

页码：

收录情况： SCI

摘要： The stability of organic acid metabolites and microbial communities structure contributed to improve the efficiency of anaerobic digestion in the large-scale biogas project. To study the relationship between the organic acid metabolites and microbial communities,three stirring speeds including 0,80 and 200 r/ min in a 4-L laboratory fermenter were set to monitor the physical and chemical qualitative changes such as volatile fatty acids during pig manure digestion. Combined with high-throughput sequencing technology,the evolution of microbial diversity and community structure in anaerobic digestion system was organically related to the metabolism of organic acids. The results indicated that,Firmicutes,Bacteroides,Proteobacteria and Euryarchaeota were the main dominant bacterial taxa in the microbial community structure. Firstly,moderate strring could improve the efficiency of anaerobic digestion and the stability of microbial community in the fermentation system in which the removal rate of total solids,volatile solids and chemical oxygen demand reached 79. 15%,63. 63% and 87. 89%,respectively. Moreover,as the main organic acid metabolites,the concentration of the acetic acid and propionic acid were positively correlated with Bacteroidetes and negatively correlated with Proteobacteria. Secondly,with high-speed stirring measure,the increased abundance of hydrogen-producing acetogens Synergistetes led to an increase in the abundance of Acidobacteria. Meanwhile, the concentration of acetic acid and propionic acid was increased by 21. 09% and 6. 44% respectively,and the abundance of hydrogenotrophic methanogens Methanocorpusculum was increased by 31.95%. Interestingly,the yield of methane had actually fallen. These data showed that excessive stirring during anaerobic digestion increased the concentrations of acetic acid and propionic acid significantly,and promoted the switchcover of methanogenic pathway from acetic acid pyrolysis pathway to less efficient acetic acid oxidation pathway. The results provided a new perspective for understanding the regulation between organic acid metabolism and methanogenic pathway in anaerobic digestion of pig manure.

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