Component Identification and Analysis of Vesicular Fluid From Swine Infected by Foot-and-Mouth Disease Virus

文献类型： 外文期刊

第一作者： Zhang, Ting

作者： Zhang, Ting;Lu, Bingzhou;Yang, Bo;Zhang, Dajun;Shi, Xijuan;Shen, Chaochao;Cui, Huimei;Yuan, Xingguo;Zhao, Dengshuai;Yang, Jinke;Hao, Yu;Chen, Xuehui;Liu, Xiangtao;Zhang, Keshan;Zheng, Haixue

作者机构：

关键词： swine; FMDV; vesicle fluid; identification and analysis; inflammation

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.471； 五年影响因子：3.821 ）

ISSN：

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： Foot-and-mouth disease (FMD) is induced by FMD virus (FMDV) and characterized by fever and vesicular (blister-like) lesions. However, the exact composition of the vesicular fluid in pigs infected with FMDV remains unclear. To identify and analyze the components of the vesicular fluid in FMDV-infected domestic pigs, the fluid was collected and subjected to mass spectrometry. Further analyses were conducted using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genome (KEGG), and protein-protein interaction (PPI). Quantitative ELISA kit for TNF-alpha, and IFN-alpha, IFN-beta, IL-6, IL-10, IL-1 beta, and IFN-gamma were used to verify the mass spectrometry results. Results showed that 937 proteins were identified in the vesicular fluid from swine after FMDV infection, and bioinformatics analysis indicated that these proteins are related to the innate immune and inflammation pathways. The levels of cytokines involved in the disease-related pathways, tumor necrosis factors, and IL-6 in the fluid samples were significantly increased. This study identified and analyzed the composition of vesicular fluid in pigs after FMD infection for the first time and provided interesting information that help understand the infection and pathogenesis mechanism of FMD. These information will eventually contribute to the prevention and control of FMD.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序